1. Microorganism and
culture medium: Lactobacillus
lactis-11, a lactic acid producing
strain, was grown on deMan Rogosa
Sharpe (MRS) [21] medium.
2. Supports:
The inorganic support materials are characterized
in Table 1.
The support materials were used after being washed with deionized water and sterilized by
autoclaving for 25 min at 1 atm and
121 C.
Table 2
|
Characterization of
support materials |
Support |
Form
|
Company
|
Mean particle diameter (mm) |
Mean pore diameter (µm) |
Specific surface area (m2
g-1) |
Porosity
(%) |
Density
(g cm-3) |
Macro-activated
carbon |
Cylinder |
Xinhuaa |
4–5 |
10–20 |
≥300 |
C50 |
1.06 |
Glass
fiberb |
Ball |
Jiajiec |
20 |
– |
– |
– |
0.21 |
Ceramic |
Bead |
Xinquand |
1–2, 5–6 |
50–100 |
≥20,
≥5 |
C53 |
0.89 |
|